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Original Paper

OX40 regulates pressure overload-induced cardiac hypertrophy and remodelling via CD4+ T-cells

Qing-Qing Wu, Yuan Yuan, Xiao-Han Jiang, Yang Xiao, Zheng Yang, Zhen-Guo Ma, Hai-Han Liao, Yuan Liu, Wei Chang, Zhou-Yan Bian, Qi-Zhu Tang
Clinical Science Oct 14, 2016, 130 (22) 2061-2071; DOI: 10.1042/CS20160074
Qing-Qing Wu
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Yuan Yuan
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Xiao-Han Jiang
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Yang Xiao
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Zheng Yang
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Zhen-Guo Ma
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Hai-Han Liao
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Yuan Liu
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Wei Chang
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Zhou-Yan Bian
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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Qi-Zhu Tang
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaCardiovascular Research Institute of Wuhan University, Wuhan 430060, PR ChinaHubei Key Laboratory of Cardiology, Wuhan 430060, PR China
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  • For correspondence: qztang@whu.edu.cn
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Abstract

OX40, which belongs to the tumour necrosis factor (TNF)-receptor family, is a costimulatory receptor that can potentiate T-cell receptor signalling on the surface of T-lymphocytes. The role of OX40 in non-immune systems, particularly the cardiovascular system, has not been defined. In the present study, we observed a noticeable increase in OX40 expression during cardiac remodelling in rodent heart. In the present study, cardiac hypertrophy was induced by aortic banding (AB) in OX40 knockout (KO) mice and wild-type (WT) mice. After 8 weeks, the OX40 KO mice showed significantly attenuated cardiac hypertrophy, fibrosis and inflammation as well as preserved cardiac function compared with the WT mice. Follow-up in vitro studies suggested that CD4+ T-lymphocyte proliferation and pro-inflammatory cytokine release were significantly decreased, whereas anti-inflammatory cytokine release was considerably increased in OX40 KO mice compared with WT mice as assessed by Cell Counting Kit-8 (CCK-8) assay and ELISA. Co-culturing neonatal rat cardiomyocytes with the activated supernatant of CD4+ T-lymphocytes from OX40 KO mice reduced the hypertrophy response. Interestingly, OX40 KO mice with reconstituted CD4+ T-lymphocytes presented deteriorated cardiac remodelling. Collectively, our data indicate that OX40 regulates cardiac remodelling via the modulation of CD4+ T-lymphocytes.

  • Ang II
  • cardiac remodelling
  • CD4+ T-lymphocytes
  • OX40
  • pressure overload
  • Abbreviations

    AB,
    aortic banding;
    ACS,
    acute coronary syndrome;
    Ang II,
    angiotensin II;
    ANP,
    atrial natriuretic peptide;
    BNP,
    β-type natriuretic peptide;
    BW,
    body weight;
    CCK,
    Cell Counting Kit;
    CSA,
    cross-sectional area;
    CTGF,
    connective tissue growth factor;
    ECM,
    cardiac extracellular matrix;
    GPCR,
    G-alpha(q) protein-coupled receptor;
    HE,
    haematoxylin–eosin;
    HF,
    heart failure;
    HW,
    heart weight;
    IFN-γ,
    interferon-gamma;
    IL,
    interleukin;
    KO,
    knockout;
    LV,
    left ventricular;
    MHC,
    myosin heavy chain;
    NRCM,
    neonatal rat cardiomyocyte;
    PSR,
    Picro-Sirius red;
    ROS,
    reactive oxygen species;
    RT-PCR,
    quantitative real-time polymerase chain reaction;
    SERCA2α,
    sarcoendoplasmic reticulum Ca2+-ATPase;
    SNP,
    single nucleotide polymorphism;
    TAC,
    transverse aortic constriction;
    TL,
    tibia length;
    TNF,
    tumour necrosis factor;
    WGA,
    wheat germ agglutinin;
    WT,
    wild-type
    • © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society
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    November 2016

    Volume: 130 Issue: 22

    Clinical Science: 130 (22)
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    OX40 regulates pressure overload-induced cardiac hypertrophy and remodelling via CD4+ T-cells
    Qing-Qing Wu, Yuan Yuan, Xiao-Han Jiang, Yang Xiao, Zheng Yang, Zhen-Guo Ma, Hai-Han Liao, Yuan Liu, Wei Chang, Zhou-Yan Bian, Qi-Zhu Tang
    Clinical Science Nov 2016, 130 (22) 2061-2071; DOI: 10.1042/CS20160074
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    OX40 regulates pressure overload-induced cardiac hypertrophy and remodelling via CD4+ T-cells
    Qing-Qing Wu, Yuan Yuan, Xiao-Han Jiang, Yang Xiao, Zheng Yang, Zhen-Guo Ma, Hai-Han Liao, Yuan Liu, Wei Chang, Zhou-Yan Bian, Qi-Zhu Tang
    Clinical Science Nov 2016, 130 (22) 2061-2071; DOI: 10.1042/CS20160074

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    Keywords

    Ang II
    cardiac remodelling
    CD4+ T-lymphocytes
    OX40
    pressure overload

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