Skip to main content

Main menu

  • Home
  • About the Journal
    • General Information
    • Scope
    • Editorial Board
    • Benefits of Publishing
    • Impact & Metrics
    • Advertising/Sponsorship
    • About the Biochemical Society
  • Current Issue
  • For Authors
    • Submit Your Paper
    • Submission Guidelines
    • Editorial Policy
    • Open Access Policy
    • Rights and Permissions
    • Biochemical Society Member Benefits
  • For Librarians
    • Subscriptions and Pricing
    • Open Access Policy
    • Clinical Science- Terms and Conditions of Usage
    • Register for Free Trial
  • For Readers
    • Rights and Permissions
    • Biochemical Society Member Benefits
    • Request a Free Trial
  • Collections
  • Help
    • Contact Us
  • Other Publications
    • NEW: Emerging Topics in Life Sciences
    • NEW: Neuronal Signaling
    • Clinical Science
    • Biochemical Journal
    • Biochemical Society Transactions
    • Bioscience Reports
    • Essays in Biochemistry
    • Biochemical Society Symposia
    • Cell Signalling Biology
    • Glossary of Biochemistry and Molecular Biology
    • The Biochemist
    • Biochemical Society

User menu

  • Log-in
  • Subscribe
  • Contact Us

Search

  • Advanced search
  • Other Publications
    • NEW: Emerging Topics in Life Sciences
    • NEW: Neuronal Signaling
    • Clinical Science
    • Biochemical Journal
    • Biochemical Society Transactions
    • Bioscience Reports
    • Essays in Biochemistry
    • Biochemical Society Symposia
    • Cell Signalling Biology
    • Glossary of Biochemistry and Molecular Biology
    • The Biochemist
    • Biochemical Society

Log-in

Sign-up for alerts  
  • My Cart
Clinical Science
Browse Archive
Advanced Search
  • Home
  • About the Journal
    • General Information
    • Scope
    • Editorial Board
    • Benefits of Publishing
    • Impact & Metrics
    • Advertising/Sponsorship
    • About the Biochemical Society
  • Current Issue
  • For Authors
    • Submit Your Paper
    • Submission Guidelines
    • Editorial Policy
    • Open Access Policy
    • Rights and Permissions
    • Biochemical Society Member Benefits
  • For Librarians
    • Subscriptions and Pricing
    • Open Access Policy
    • Clinical Science- Terms and Conditions of Usage
    • Register for Free Trial
  • For Readers
    • Rights and Permissions
    • Biochemical Society Member Benefits
    • Request a Free Trial
  • Collections
  • Help
    • Contact Us

Research article

Hyperbaric oxygen activates discoidin domain receptor 2 via tumour necrosis factor-α and the p38 MAPK pathway to increase vascular smooth muscle cell migration through matrix metalloproteinase 2

Kou-Gi Shyu, Bao-Wei Wang, Hang Chang
Clinical Science Mar 02, 2009, 116 (7) 575-583; DOI: 10.1042/CS20080215
Kou-Gi Shyu
Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, TaiwanGraduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • View author's works on this site
Bao-Wei Wang
Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, TaiwanSchool of Medicine, Fu-Jen Catholic University, Taipei County 24205, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • View author's works on this site
Hang Chang
Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • View author's works on this site
  • For correspondence: m002018@ms.skh.org.tw
  • Article
  • Figures
  • Info & Metrics
  • Supplementary Data
  • PDF
Loading

This article has been retracted. Please see:

  • Retraction: Hyperbaric oxygen activates discoidin domain receptor 2 via tumour necrosis factor-α and the p38 MAPK pathway to increase vascular smooth muscle cell migration through matrix metalloproteinase 2

Abstract

DDR2 (discoidin domain receptor 2) regulates collagen turnover mediated by SMCs (smooth muscle cells) in atherosclerosis. HBO (hyperbaric oxygen) has been used in medical practice; however, the molecular mechanism of the beneficial effects of HBO is poorly understood. Furthermore, the effect of HBO on DDR2 has not been reported previously. In the present study, we investigated the cellular and molecular mechanisms of DDR2 regulation by HBO in VSMCs (vascular SMCs). Cells were exposed to 2.5 ATA (atmosphere absolute) of oxygen in a hyperbaric chamber. DDR2 protein (3.63-fold) and mRNA (2.34-fold) expression were significantly increased after exposure to 2.5 ATA HBO for 1 h. Addition of SB203580 and p38 MAPK (mitogen-activated protein kinase) siRNA (small interfering RNA) 30 min before HBO inhibited the induction of DDR2 protein. HBO also significantly increased DNA–protein binding activity of Myc/Max. Addition of SB203580 and an anti-TNF-α (tumour necrosis factor-α) monoclonal antibody 30 min before HBO abolished the DNA–protein binding activity induced by HBO. HBO significantly increased the secretion of TNF-α from cultured VSMCs. Exogenous addition of TNF-α significantly increased DDR2 protein expression, whereas anti-TNF-α and anti-(TNF-α receptor) antibodies blocked the induction of DDR2 protein expression. HBO significantly increased VSMC migration and proliferation, whereas DDR2 siRNA inhibited the migration induced by HBO. HBO increased activated MMP2 (matrix metalloproteinase 2) protein expression, and DDR2 siRNA abolished the induction of activated MMP2 expression induced by HBO. In conclusion, HBO activates DDR2 expression in cultured rat VSMCs. HBO-induced DDR2 is mediated by TNF-α and at least in part through the p38 MAPK and Myc pathways.

  • angiogenesis
  • discoidin domain receptor 2 (DDR2)
  • hyperbaric oxygen
  • matrix metalloproteinase (MMP)
  • p38 mitogen-activated protein kinase (p38 MAPK)
  • smooth muscle cell

Abbreviations: Ang2, angiopoietin 2; ATA, atmosphere absolute; DDR, discoidin domain receptor; EMSA, electrophoretic mobility-shift assay; HBO, hyperbaric oxygen; MAPK, mitogen-activated protein kinase; MMP, matrix metalloproteinase; RNAi, RNA interference; siRNA, small interfering RNA; SMC, smooth muscle cell; TNF-α, tumour necrosis factor-α; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling; VEGF, vascular endothelial growth factor; VSMC, vascular SMC

  • © The Authors Journal compilation © 2009 Biochemical Society
View Full Text

Log in using your username and password

Enter your Clinical Science username.
Enter the password that accompanies your username.
Forgot your user name or password?

Log in through your institution

You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.
If your organization uses OpenAthens, you can log in using your OpenAthens username and password. To check if your institution is supported, please see this list. Contact your library for more details.
Previous ArticleNext Article
Back to top

April 2009

Volume: 116 Issue: 7

Clinical Science: 116 (7)
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author

Actions

Email

Thank you for your interest in spreading the word about Clinical Science.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Hyperbaric oxygen activates discoidin domain receptor 2 via tumour necrosis factor-α and the p38 MAPK pathway to increase vascular smooth muscle cell migration through matrix metalloproteinase 2
(Your Name) has forwarded a page to you from Clinical Science
(Your Name) thought you would like to see this page from the Clinical Science web site.
Share
Hyperbaric oxygen activates discoidin domain receptor 2 via tumour necrosis factor-α and the p38 MAPK pathway to increase vascular smooth muscle cell migration through matrix metalloproteinase 2
Kou-Gi Shyu, Bao-Wei Wang, Hang Chang
Clinical Science Apr 2009, 116 (7) 575-583; DOI: 10.1042/CS20080215
del.icio.us logo Digg logo Reddit logo Technorati logo Twitter logo CiteULike logo Facebook logo Mendeley logo
Citation Tools
Hyperbaric oxygen activates discoidin domain receptor 2 via tumour necrosis factor-α and the p38 MAPK pathway to increase vascular smooth muscle cell migration through matrix metalloproteinase 2
Kou-Gi Shyu, Bao-Wei Wang, Hang Chang
Clinical Science Apr 2009, 116 (7) 575-583; DOI: 10.1042/CS20080215

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print
Alerts

Please log in to add an alert for this article.

Request Permissions
Save to my folders

View Full PDF

 Open in Utopia Docs
  • Tweet Widget
  • Facebook Like

Jump To

  • Article
    • Abstract
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • FUNDING
    • References
  • Figures
  • Info & Metrics
  • Supplementary Data
  • PDF

Keywords

angiogenesis
discoidin domain receptor 2 (DDR2)
hyperbaric oxygen
matrix metalloproteinase (MMP)
p38 mitogen-activated protein kinase (p38 MAPK)
smooth muscle cell

Related Articles

Cited By...

  • Portland Press Homepage
  • Publish With Us
  • Advertising
  • Technical Support
  • Clinical Science
  • Biochemical Journal
  • Essays in Biochemistry
  • Biochemical Society Transactions
  • Biochemical Society Symposia
  • Bioscience Reports
  • Emerging Topics in Life Sciences
  • Neuronal Signaling
  • Cell Signalling Biology

Portland Press Limited
Charles Darwin House
12 Roger Street
London WC1N 2JU
Tel: +44(0) 20 7685 2410
Fax: +44(0) 20 7685 2469
Email: editorial@portlandpress.com

The Biochemical Society